Minute™ Protein/Nucleic Acid Extraction Kit from Gels
Passive elution and electro elution are the most common methods for extracting proteins and/or nucleic acid from polyacrylamide/agarose gels. Protein extraction by passive elution usually takes overnight incubation. Eluted proteins are significantly diluted and require further concentration. Protein extraction by electro elution is faster (30 min to 2 h) than passive elution but it requires a special elution device that works less effectively for larger proteins (>70 Kda). The electro buffer usually contains detergent and other chemicals at a concentration that may interfere with downstream applications. Here we feature a rapid and instrument-free protein/nucleic acid extraction kit. Proteins/nucleic acid can be extracted from gels in <10 min with high yield. The elution buffer can be a detergent-containing buffer or pure water depending upon methods of gel staining. The elution volume is between 10 to 200 µl. Multiple gel pieces can be processed in a single tube and the final protein concentration is relatively high.
Price:$120 (20 preps)(Free sample not available for this product)